Long-term supplementation with young coconut juice help prevent bone loss of orchidectomized rats by increasing connectivity density, percentage bone volume and osteoblast-/osteocyte-(ERα-/ ERβ-) immunoreactive cells of the L5 vertebra and femur

Adult male rats were orchidectomized (ORX) and treated with 17β-estradiol or young coconut juice (YCJ) for ten weeks. Their femur L5 vertebra dissected, the percentage of bone volume was measured using a Micro-CT scan. Immunohistochemistry used to detect ERα ERβ determine if osteoporotic protective effects YCJ due attachment active components(s) estrogen receptors. It found that both in osteocytes osteoblasts, but not osteoclasts. Compared normal rats, number osteoblast- osteocyte- reactive cells significantly reduced ORX rats. Those numbers restored by injecting estradiol benzoate feeding which dose-related. Significant correlations detected between osteoblast-/osteocyte- (ERα-/ERβ)-reactive vs. serum E2 level. The optimal dose this study 10 mL/kg BW/ day ERα-of osteoblasts 40 BW/day ERβ-of osteocytes. results suggest may be as efficient attenuating osteoporosis, probably being selective receptor moderator.